Cryo-EM Workshop 2025: "I have my data set — Now what?"
Single-particle Cryo-EM is a powerful structural technique that calculates 3D structures of biological specimens. Recent technological developments in microscopes, cameras and computation have led to a “resolution revolution” in the field, and it is now routine to determine 3D structures under 3Å resolution.
For our 2025 single-particle workshop, we will focus exclusively on approaches to deal with conformational/compositional heterogeneity. We will provide users with particle stacks used in RELION, cryoDRGN and cryoSPARC. This is a hands-on class, where participants will work with instructors to learn how to independently run these software packages on a machine in the cloud.
Instruction is geared toward participants with some experience processing Cryo-EM data. We will not be covering initial movie alignment, CTF estimation, and particle selection steps.
Applications are due by 5:00 p.m. ET on March 21.
Who Should Attend
Graduate students, post-doctoral fellows, faculty, staff, and non-academics (such as industry) with cryo-EM experience and at least one cryo-EM dataset for processing. Priority will be given to participants who are learning cryo-EM and are actively analyzing datasets. We expect participants to have a basic knowledge of cryo-EM and a familiarity with cryo-EM software.