LSI Seminar Series: Jiefu Li, Ph.D., HHMI Janelia Research Campus
Spatially-resolved proteomics of the brain: Methods, proteomes and mechanistic discoveries
Molecular compartmentalization is vital for cellular physiology. For instance, proteins for intercellular adhesion and communication are localized to the cell surface while many energy-producing enzymes stay in the mitochondrion. High-resolution, proteome-wide mapping of protein localization is of core importance for understanding cellular organization and processes.
In this talk, Jiefu Li, Ph.D., will describe an end-to-end pipeline for in situ cell-surface proteomics, from chemical and transgenic tools to a user-friendly platform for data analysis, and present how in situ cell-surface proteomics of developing brains discovers “outside-the-box” regulators of neuronal wiring. Moreover, in vivo interactome mapping of key synaptic organizers uncovers the cellular and molecular mechanisms of synaptic matching and maturation and highlights the RhoGAP/RhoGEF as a signaling hub for synaptic development.
Speaker
Jiefu Li is a Group Leader of 4D Cellular Physiology at the HHMI Janelia Research Campus. The Li lab develops methods for analyzing cell-surface signals at systems, cellular and molecular levels and studies cell-surface signaling in neurobiology and physiology.