Backward movement of daughter vacuoles in stabilized Vac17 mutant cells. VAC17 or stabilized vac17 mutant plasmid was introduced into a vac17Δ strain containing genomically integrated MYO2-GFP. The transformants were labeled with FM4-64 for 1.5 hours, and then chased for 1 hour. Cells were mounted on a thin layer of 25% gelatin (in SC-URA media) and incubated in a 30°C temperature champer while images were acquired every 3 min. Overlay images indicate relative position of vacuoles (red) and Myo2p (green). Scale bar, 5 µm.